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![]() | Immunoblotting Whole cell extract of HEK-293T expressing human recombinant EDEM2 was separated on SDS-PAGE and probed with Rabbit Anti-EDEM2 (N-terminal) (Cat. No. E9906). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 5 μg/mL 2. Antibody 5 μg/mL + EDEM2 immunizing peptide (human, 22-38) |
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E9906
Sigma
Anti-EDEM2 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-ER degradation enhancer, mannosidase alpha-like 2 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 22-38 of human EDEM2, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and 2 amino acids in rat EDEM2. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~70 kDa |
| species reactivity | mouse, human, rat (predicted) |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2 |
| indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells | |
| Gene Information | human ... EDEM2(55741) |
| rat ... Edem2(296304) | |
| mouse ... Edem2(108687) | |
| shipped in | dry ice |
| storage temp. | −20°C |







