E9906

Immunoblotting
Whole cell extract of HEK-293T expressing human recombinant EDEM2 was separated on SDS-PAGE and probed with Rabbit Anti-EDEM2 (N-terminal) (Cat. No. E9906). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody 5 μg/mL
2. Antibody 5 μg/mL + EDEM2 immunizing peptide (human, 22-38)

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E9906

Sigma

 

Anti-EDEM2 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym:Anti-ER degradation enhancer, mannosidase alpha-like 2

References

Reviews

Description

Immunogensynthetic peptide corresponding to amino acids 22-38 of human EDEM2, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and 2 amino acids in rat EDEM2.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formaffinity isolated antibody
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt ~70 kDa
species reactivitymouse, human, rat (predicted)
concentration~1.0 mg/mL
application(s)immunoblotting: 0.5-1 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2
 indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
Gene Informationhuman ... EDEM2(55741)
 rat ... Edem2(296304)
 mouse ... Edem2(108687)
shipped indry ice
storage temp.−20°C

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